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SRX23971030: DNA methylation sequencing of Alligator sinensis:Muscle of hindlimb
1 ILLUMINA (Illumina HiSeq 2500) run: 241.3M spots, 72.4G bases, 22.5Gb downloads

Design: After the sample DNA test was qualified, a certain proportion of lambda DNA was added as Bisulfite conversion test, and Covaris M220 was used to randomly interrupt genomic DNA to 200-400bp. End repair of broken DNA fragments, adding A tail, and methylated sequencing splices; Then Bisulfite treatment (using EZ DNA Methylation Gold Kit, Zymo Research) was performed. After treatment, the unmethylated C became U (T after PCR amplification), while the methylated C remained unchanged, and finally PCR amplification was performed. Get the final WGBS library
Submitted by: Wuhu Institute of Technology
Study: Genome-wide DNA methylation of hindlimb muscles of Chinese alligator
show Abstracthide Abstract
We employed genome-wide bisulfite sequencing (WGBS) to generate single-base resolution DNA methylation maps of the hindlimb muscles in hibernating and active Chinese alligators, aiming to elucidate the regulatory mechanisms of DNA methylation in hibernation adaptation of Chinese alligators. The WGBS technique is widely regarded as one of the most effective methods for detecting DNA methylation. The findings from this study will provide a fundamental foundation for gaining further insights into the adaptive regulatory mechanisms of hindlimb muscles in Chinese alligators during hibernation.
Sample:
SAMN40500219 • SRS20769732 • All experiments • All runs
Library:
Name: HM2_L3
Instrument: Illumina HiSeq 2500
Strategy: Bisulfite-Seq
Source: GENOMIC
Selection: 5-methylcytidine antibody
Layout: PAIRED
Runs: 1 run, 241.3M spots, 72.4G bases, 22.5Gb
Run# of Spots# of BasesSizePublished
SRR28365923241,270,53572.4G22.5Gb2024-03-17

ID:
32276960

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